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cd11b fitc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd11b fitc
    Cd11b Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+c+fitc/CD11b%2Fc+Antibody%2C+anti-rat%2C+REAfinity/pm41981447-143-19-29
    Average 98 stars, based on 26 article reviews
    cd11b fitc - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Novel celecoxib analogues inhibit glial production of prostaglandin E2, nitric oxide, and oxygen radicals reverting the neuroinflammatory responses induced by misfolded prion protein fragment 90-231 or lipopolysaccharide.
    Article Snippet: Please cite this article as: Villa Valentina, Thellung Stefano, Bajetto Adriana, Gatta Elena, Robello Mauro, Novelli Federica, Tasso Bruno, Tonelli Michele, Florio Tullio.NOVEL CELECOXIB ANALOGUES INHIBIT GLIAL PRODUCTION OF PROSTAGLANDIN E2, NITRIC OXIDE, AND OXYGEN RADICALS REVERTING THENEUROINFLAMMATORYRESPONSES INDUCEDBYMISFOLDEDPRION PROTEIN FRAGMENT 90-231 OR LIPOPOLYSACCHARIDE.Pharmacological Research http://dx.doi.org/10.1016/j.phrs.2016.09.010

    Article Title: Fatty acid suppression of glial activation prevents central neuropathic pain after spinal cord injury.
    Article Snippet: .. Cells were incubated with CD11b/c-FITC (clone-REA325) and CD86-PE (clone-24F) (Miltenyi Biotec) at 1: 10 dilution (106 cells/50 mL) for 45 minutes at room temperature. .. Samples were analysed using BD Fortessa (a minimum of 10,000 events) and FlowJo software.

    Purification:

    Article Title: miR-204 inhibits the osteogenic differentiation of mesenchymal stem cells by targeting bone morphogenetic protein 2
    Article Snippet: The cells were centrifuged at 750 × g for 5 min at room temperature after digestion using 0.25% trypsin-EDTA (Gibco; Thermo Fisher Scientific, Inc.) at 37°C for 5 min in an incubator (Thermo Fisher Scientific, Inc.). .. Purified BMSCs (2×10 7 cells/ml) were seeded in 6-well plates and cluster of differentiation (CD) 90-allophycocyanin (APC; 1:20; cat. no. 17-5900-42; Invitrogen; Thermo Fisher Scientific, Inc.), CD45-phycoerythrin/cyanine7 (PE/CY7; 0.1 mg/ml; cat. no. 1660-17, SouthernBiotech) and CD11b/c-FITC (30 μg/ml, cat. no. 130-105-273; Miltenyi Biotec, Inc.) antibodies were used to stain the samples in a final volume of 100 μl at 4°C for 20 min. Then, BMSCs in solution were identified via flow cytometry and analyzed with CellQuest pro software version 5.1 (BD Biosciences), by detecting the absorbance at 647 nm for CD90-APC, 532 nm for CD45-PE/CY7 and 488 nm for CD11b/c-FITC. .. BMSCs were seeded in 75-mm culture dishes at 3.5×10 5 cells/dish and incubated for 24 h. miR-204 agomir (5′-UUCCCUUUGUCAUCCUAUGCC-3′), miR-204 antagomir (5′-AGGCAUGGAUGACAAAGGGAA-3′) and negative control (NC, 5′-CAGUACUUUUGUGUAGUACAA-3′) sequences were purchased from Guangzhou RiboBio Co., Ltd. pLVX-AcGFP-C1-BMP2 was constructed by cloning amplified BMP2 into pLVX-AcGFP-C1 (Sangon Biotech Co., Ltd.). pLVX-AcGFP-C1 was used as the negative control.

    Staining:

    Article Title: miR-204 inhibits the osteogenic differentiation of mesenchymal stem cells by targeting bone morphogenetic protein 2
    Article Snippet: The cells were centrifuged at 750 × g for 5 min at room temperature after digestion using 0.25% trypsin-EDTA (Gibco; Thermo Fisher Scientific, Inc.) at 37°C for 5 min in an incubator (Thermo Fisher Scientific, Inc.). .. Purified BMSCs (2×10 7 cells/ml) were seeded in 6-well plates and cluster of differentiation (CD) 90-allophycocyanin (APC; 1:20; cat. no. 17-5900-42; Invitrogen; Thermo Fisher Scientific, Inc.), CD45-phycoerythrin/cyanine7 (PE/CY7; 0.1 mg/ml; cat. no. 1660-17, SouthernBiotech) and CD11b/c-FITC (30 μg/ml, cat. no. 130-105-273; Miltenyi Biotec, Inc.) antibodies were used to stain the samples in a final volume of 100 μl at 4°C for 20 min. Then, BMSCs in solution were identified via flow cytometry and analyzed with CellQuest pro software version 5.1 (BD Biosciences), by detecting the absorbance at 647 nm for CD90-APC, 532 nm for CD45-PE/CY7 and 488 nm for CD11b/c-FITC. .. BMSCs were seeded in 75-mm culture dishes at 3.5×10 5 cells/dish and incubated for 24 h. miR-204 agomir (5′-UUCCCUUUGUCAUCCUAUGCC-3′), miR-204 antagomir (5′-AGGCAUGGAUGACAAAGGGAA-3′) and negative control (NC, 5′-CAGUACUUUUGUGUAGUACAA-3′) sequences were purchased from Guangzhou RiboBio Co., Ltd. pLVX-AcGFP-C1-BMP2 was constructed by cloning amplified BMP2 into pLVX-AcGFP-C1 (Sangon Biotech Co., Ltd.). pLVX-AcGFP-C1 was used as the negative control.

    Flow Cytometry:

    Article Title: miR-204 inhibits the osteogenic differentiation of mesenchymal stem cells by targeting bone morphogenetic protein 2
    Article Snippet: The cells were centrifuged at 750 × g for 5 min at room temperature after digestion using 0.25% trypsin-EDTA (Gibco; Thermo Fisher Scientific, Inc.) at 37°C for 5 min in an incubator (Thermo Fisher Scientific, Inc.). .. Purified BMSCs (2×10 7 cells/ml) were seeded in 6-well plates and cluster of differentiation (CD) 90-allophycocyanin (APC; 1:20; cat. no. 17-5900-42; Invitrogen; Thermo Fisher Scientific, Inc.), CD45-phycoerythrin/cyanine7 (PE/CY7; 0.1 mg/ml; cat. no. 1660-17, SouthernBiotech) and CD11b/c-FITC (30 μg/ml, cat. no. 130-105-273; Miltenyi Biotec, Inc.) antibodies were used to stain the samples in a final volume of 100 μl at 4°C for 20 min. Then, BMSCs in solution were identified via flow cytometry and analyzed with CellQuest pro software version 5.1 (BD Biosciences), by detecting the absorbance at 647 nm for CD90-APC, 532 nm for CD45-PE/CY7 and 488 nm for CD11b/c-FITC. .. BMSCs were seeded in 75-mm culture dishes at 3.5×10 5 cells/dish and incubated for 24 h. miR-204 agomir (5′-UUCCCUUUGUCAUCCUAUGCC-3′), miR-204 antagomir (5′-AGGCAUGGAUGACAAAGGGAA-3′) and negative control (NC, 5′-CAGUACUUUUGUGUAGUACAA-3′) sequences were purchased from Guangzhou RiboBio Co., Ltd. pLVX-AcGFP-C1-BMP2 was constructed by cloning amplified BMP2 into pLVX-AcGFP-C1 (Sangon Biotech Co., Ltd.). pLVX-AcGFP-C1 was used as the negative control.

    Software:

    Article Title: miR-204 inhibits the osteogenic differentiation of mesenchymal stem cells by targeting bone morphogenetic protein 2
    Article Snippet: The cells were centrifuged at 750 × g for 5 min at room temperature after digestion using 0.25% trypsin-EDTA (Gibco; Thermo Fisher Scientific, Inc.) at 37°C for 5 min in an incubator (Thermo Fisher Scientific, Inc.). .. Purified BMSCs (2×10 7 cells/ml) were seeded in 6-well plates and cluster of differentiation (CD) 90-allophycocyanin (APC; 1:20; cat. no. 17-5900-42; Invitrogen; Thermo Fisher Scientific, Inc.), CD45-phycoerythrin/cyanine7 (PE/CY7; 0.1 mg/ml; cat. no. 1660-17, SouthernBiotech) and CD11b/c-FITC (30 μg/ml, cat. no. 130-105-273; Miltenyi Biotec, Inc.) antibodies were used to stain the samples in a final volume of 100 μl at 4°C for 20 min. Then, BMSCs in solution were identified via flow cytometry and analyzed with CellQuest pro software version 5.1 (BD Biosciences), by detecting the absorbance at 647 nm for CD90-APC, 532 nm for CD45-PE/CY7 and 488 nm for CD11b/c-FITC. .. BMSCs were seeded in 75-mm culture dishes at 3.5×10 5 cells/dish and incubated for 24 h. miR-204 agomir (5′-UUCCCUUUGUCAUCCUAUGCC-3′), miR-204 antagomir (5′-AGGCAUGGAUGACAAAGGGAA-3′) and negative control (NC, 5′-CAGUACUUUUGUGUAGUACAA-3′) sequences were purchased from Guangzhou RiboBio Co., Ltd. pLVX-AcGFP-C1-BMP2 was constructed by cloning amplified BMP2 into pLVX-AcGFP-C1 (Sangon Biotech Co., Ltd.). pLVX-AcGFP-C1 was used as the negative control.



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